Monday, January 30, 2017

pGLO Lab


Plate
Number of Colonies
Color of colonies under room light
Color of colonies under   UV light
- pGLO LB
0
none
none
- pGLO LB/amp
none
none
none
+ pGLO LB/amp
2
white/clear
white/clear
+ pGLO LB/amp/ara
At least 4
white/clear
bright green

          2. The two new traits that the transformed bacteria have are: the ability to glow under UV light and ampicillin resistance.

          3.  I think there were millions of bacteria in the 100 uL of bacteria that was spread on each plate because bacteria are very small in size, many bacteria could be found in a little amount of E.coli.

          4. The rold of arabinose in the plates was to activate the gene that makes the bacteria glow green when UV light is present.

         5.  a)GFP can be used to dye cancer cells and shbow images of them
              b) GFP can show if the bacteria took a new gene properly
              c) GFP can show if someone had a drug overdose
         
         6. Genetic engineering can be used to create genetically modified crops. Corn, papaya, cotton, and soy are all at a high risk for being genetically modified. Crops are genetically modified to increase the resistance against a disease in a crop.




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